Endo H

Storage Solution: 500U/μL EndoH、20 mM Tris-HCl、50 mM NaCl、5 mM EDTA (pH 7.5@ 25°C)

10*Denaturing Buffer: 5% SDS、400 mM DTT

10*Reaction Buffer: 500 mM sodium acetate(pH 6 @ 25°C)

1. Combine 1-20 μg of glycoprotein, 1 μl of 10*Denaturing Buffer and H20 (if necessary) to make a 10 μl total reaction volume.

2. Denature glycoprotein by heating raection at 100°C for 10 minutes.

3. Make a total reaction volume of 20 μl by adding 2 μl of 10*Reaction Buffer, H20 and 1-5 μl Endo H.

4. Incubate reaction at 37°C for 1 hour.

Please avoid repeated freeze-thaw cycles.