Storage
Solution : 200U/ul Endo S、20
mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. (pH 7.5 @ 25°C)
10*Reaction Buffer：50mM CaCl2、500 mM sodium acetate (pH 5.5 @ 25°C)
操作步骤
1.
Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total
reaction volume.
2.
Add 1 µl Endo S.
3. Incubate reaction at 37°C for 1 hour
注意事项
Please avoid repeated freeze-thaw cycles.
产品数据
生物活性
The
experimental design added 10 U, 2 U, 1 U, 0.5 U, 0.4U and 0.25 U enzymes into
the 20 ul reaction system to investigate the effect of Endo S on the digestion
of human IgG.
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营业执照（三证合一） 
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