IL-4 Protein, Human

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells, the EC50 for this effect is less than 0.2ng/ml.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is less than 0.2ng/ml.

1μg (R: reducing condition, N: non-reducing condition).

Primary antibody: CD209 gamma Recombinant Rabbit mAb (S0B1390) at 1/1000 dilution

Lane 1: untreated THP-1 whole cell lysate 20 µg

Lane 2: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

Lane 3: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

Lane 4: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

Lane 5: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 46 kDa

Observed MW: 45~55 kDa

Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 2.0μg/mL (100μL/well) can bind IL-4R alpha/CD124 Fc Chimera Protein, Cynomolgus (Cat. No. UA010387) with EC50 of 1.97-3.24 ng/mL.

Immobilized IL-4, Human (Cat. No. UA040026) at 2.0μg/mL (100μL/well) can bind IL-4Rα/CD124 His Tag, Cynomolgus (Cat. No. UA010379) with EC50 of 42.22-60.87 ng/mL.

Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 5.0μg/mL (100μL/well) can bind IL-4R alpha/CD124 His Tag Protein, Human (Cat. No. UA010382) with EC50 of 13.41-18.20ng/mL.

Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 5.0μg/mL (100μL/well) can bind Biotinylated IL-4R alpha/CD124 Avi&His Tag Protein, Human (Cat. No. UA010671) with EC50 of 27.67-45.87ng/mL.