IFN-α 2b Protein, Human

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC₅₀ for this effect is less than 60 pg/ml.

Measured by a cytotoxicity assay using TF-1 Cells, The ED50 for this effect is less than 0.8ng/ml.

2μg (R: reducing condition, N: non-reducing condition).

>90% as determined by RP-HPLC.

Primary antibody: IFIT1 Recombinant Rabbit mAb (S0B1242) at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml IFN-α2b(UA040399) for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa