IL-6 Protein, Human

Measured in a cell proliferation assay using TF-1 cells, the EC50 for this effect is less than 0.2ng/ml.

1μg (R:reducingcondition;N:non-reducingcondition).

Immobilized IL-6, Human (Cat. No. UA040052) at 5.0μg/mL (100μL/well) can bind IL-6R alpha/CD126 Fc Chimera, Human (Cat. No. UA010448) with EC50 of 3.04-3.92 ng/mL.

Immobilized IL-6 Protein, Human (Cat. No. UA040052) at 2.0μg/mL (100μL/well) can bind Biotinylated IL-6 R alpha/CD126 Fc&Avi Tag Protein, Human (Cat. No. UA011222) with EC50 of 8.02-11.36ng/mL.

Immobilized Human IL-6 (Cat. No. UA040052) at 5.0μg/mL (100μL/well) can bind Biotinylated IL-6R alpha/CD126 Avi&His Tag Protein, Human (Cat. No. UA011248) with EC50 of 78.37-140.2ng/mL.

Protein A Chip captured IL-6R alpha/CD126 Fc Chimera, Human (Cat. No. UA010448), can bind IL-6, Human (Cat. No. UA040052) with an affinity constant of 8.51nM as determined in SPR assay.