2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with 0.01ug PE-Labeled CD19 His Tag Protein, Human, (Cat. No. UA011320) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity. 2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
评论(0)