Endo F1

UA070121: 10U/µL EndoF1 in 20mM Tris, pH7.5 (@ 25°C)
10*Reaction buffer:500 mM Sodium phosphate, pH 5.5 (@ 25°C)

1.Take up to 200µg of glycoprotein or glycopeptide sample, adjust to 43µl with purified water;
2.Add 5µl of 10× reaction buffer;
3.Add 2µl EndoF1 to a total volume of 50µl, mix gently;
4. React for 1 hour at 37°C.

1. For different glycoprotein samples, the optimal enzyme concentration and reaction time need to be mapped experimentally;

2. Higher enzyme concentrations increase the digestion efficiency of individual glycoproteins and need to be optimized accordingly;

3. SDS and DTT will limit enzyme activity;

4. The final concentration of the enzyme after dissolution will be stored at -20℃ for storage after dispensing as needed.