Technical Articles

This article systematically elucidates the detection principle of AMP quantitative assay based on chemiluminescence, which converts AMP into ATP through coupled enzymatic reactions and generates light signals using luciferase. It also analyzes its versatile application value in various enzymatic systems, including cyclic nucleotide phosphodiesterase, DNA ligase, and E3 ubiquitin ligase.

This article focuses on the central role of adenosine monophosphate (AMP) as a key metabolite and product of multiple enzymatic reactions, systematically elaborating on the principles, advantages, and application scope of AMP quantitative detection technology based on the chemiluminescence method. It analyzes its unique value in enzyme activity determination, inhibitor screening, and mechanistic studies.