Technical Articles

Osteoclasts are the only multinucleated giant cells in the human body capable of bone resorption, and their differentiation and maturation are precisely regulated by various cytokines. Human peripheral blood CD14+ monocytes can be directionally differentiated into mature osteoclasts with bone resorption activity under the synergistic induction of macrophage colony-stimulating factor and receptor activator of nuclear factor kappa-B ligand. This in vitro induction system has become a core experimental platform for bone metabolism disease research, drug screening, and mechanism analysis.