Technical Articles

This article addresses the challenges of intraoperative tumor identification in colorectal cancer surgery, systematically elucidating the principles and advantages of near-infrared fluorescence imaging technology as it evolves from NIR-I to NIR-II. It analyzes the molecular basis of CEACAM5 as a specific imaging target for colorectal cancer and its significant expression differences between tumor and normal tissues, explores the clinical translation potential of this targeted imaging strategy in achieving precise tumor resection, and introduces the application of fluorescently labeled recombinant proteins in target validation and imaging research.