Technical Articles

GPC3 is a heparan sulfate proteoglycan located on the cell membrane surface and is a liver cancer-specific associated antigen. This protein consists of a complex glycoconjugate formed by the covalent linkage of proteins, lipids, and sugars, and is anchored to the cell membrane surface via a glycosylphosphatidylinositol (GPI) anchor.

IdeZ (Immunoglobulin G-degrading enzyme of Streptococcus zooepidemicus) is a bacterial protease that specifically cleaves IgG antibodies. In recent years, it has attracted much attention for its unique applications in antibody engineering, gene therapy, and immunological research. This article systematically reviews the molecular properties, mechanisms of action, application progress, and future challenges of IdeZ, with a focus on exploring its potential in overcoming neutralizing antibody barriers in AAV gene therapy, constructing multispecific antibodies, and treating autoimmune diseases