Technical Articles

The SCF complex consists of two pre assembled sub complexes: one is the CUL1-RBX1 core catalytic module, where CUL1 serves as the scaffold protein and RBX1 is responsible for recruiting E2 ubiquitin binding enzyme; The second is the SKP1 Fbp subunit, where SKP1 acts as a linker protein and the F box protein directly recognizes and binds to degradation determinants (degron) on specific substrates.

The SCF (SKP1-CUL1-F-box protein) complex, as a core member of the Cullin RING ligase (CRL) superfamily, is one of the most widely functional E3 ubiquitin ligases in eukaryotic cells. It selectively recognizes and ubiquitinates target proteins, regulating key biological processes such as cell cycle progression, signal transduction, and stress response.