Technical Articles

This article focuses on the molecular characteristics and biological functions of epidermal growth factor (EGF), systematically elaborating its core mechanism of activating EGFR to initiate intracellular signaling cascades and promote epidermal cell proliferation and migration. It also analyzes the significant therapeutic effects of EGF in wound healing and its limitations in non-invasive skincare.

This article systematically elaborates on the molecular characteristics and mechanisms of action of keratinocyte growth factor (KGF/FGF-7) as a unique member of the fibroblast growth factor family, analyzes its biological function in specifically regulating epithelial cell proliferation and differentiation through paracrine signaling, and explores its critical role in tissue injury repair and clinical translation potential.

This article systematically elaborates on the central role of the phosphatidylinositol 3-kinase (PI3K) signaling pathway in cellular regulation, analyzes the design principles and technical advantages of ADP detection-based kinase activity assays, and explores the application strategies of biotinylated substrates in PI3K activity analysis.

This study, based on a mouse model of aortic constriction, systematically elucidates the critical role of myeloid cell-specific ErbB3 signaling in the early adaptation of non-ischemic cardiomyopathy. It focuses on analyzing the mechanism by which this signaling pathway mediates cardiac myeloid cell recruitment and promotes non-immune cell proliferation in male mice, while revealing the sex-specific characteristics of acute heart failure induced by ErbB3 signaling deficiency.

Soquelitinib (CPI-818) has advanced to global Phase III trials for PTCL and Phase II for AD, while the first ITK PROTAC (BSJ-05-037 from Peking University) and Pfizer's dual-target topical inhibitor PF-07245303 have also been reported. These developments indicate that ITK is gradually transitioning from an "early-stage target" to the "clinical validation phase"—for teams still evaluating project initiation or building screening platforms, now may be an opportune time to make strategic moves.

This article systematically elaborates on the molecular structural characteristics of HER3 as a unique member of the HER protein family and the biological basis of its lack of kinase activity, analyzes its important role in the HER2 oncogenic mechanism and the mechanisms by which its overexpression leads to resistance in targeted therapies, reviews the challenges in the clinical translation of HER3-targeting monoclonal antibodies, and on this basis, introduces the application value of HER3 recombinant proteins in drug development.

This article systematically elucidates the molecular basis of CRBN and DDB1 forming a functional complex, analyzes its core role as the substrate recognition module of the CUL4-RING E3 ubiquitin ligase complex, and explores the pivotal position of this complex in the development of PROTACs and molecular glue drugs.

Based on the clinical treatment challenges of rhabdomyosarcoma (RMS), this article systematically elaborates on the high expression characteristics of fibroblast growth factor receptor 4 (FGFR4) in RMS and other malignant tumors, as well as its theoretical basis as an immunotherapy target. It analyzes the antitumor activity of FGFR4-targeted antibody-drug conjugates (ADCs) in preclinical studies and their potential advantages compared to CAR-T cell therapy.

This article systematically elaborates on the molecular structural characteristics of epidermal growth factor (EGF), its historical discovery, and its mechanisms of action in human skin repair and anti-aging. On this basis, it further analyzes the molecular features of chicken-derived EGF and introduces research progress in its roles in avian intestinal development, immune enhancement, appetite regulation, and follicle development.

This article systematically elaborates on the molecular structural characteristics of prolactin (PRL) and its synthetic sources in various extra-pituitary tissues, analyzes the molecular diversity of its receptor PRLR, signal transduction mechanisms, and pleiotropic roles in the immune system, reproductive system, and cancer development. On this basis, it introduces the detection and application value of soluble PRLR recombinant protein in related research.