Technical Articles

This article systematically elucidates the central role of fibroblast growth factor (FGF) family members in cell culture and the technical challenges posed by their thermal instability. It highlights the principles of thermally stable FGF technology in enhancing stability and bioactivity through protein engineering, and analyzes the unique application value of FGF-10 as a key member in organoid research.

This paper systematically elucidates the molecular mechanisms by which Noggin acts as an antagonist of the bone morphogenetic protein (BMP) signaling pathway and its central role in regulating cell proliferation and differentiation. It introduces a Noggin activity detection method based on the BMP reporter gene system and highlights its critical application value in the long-term culture of organoids.

This article focuses on the in vitro construction and cultivation of mouse lung organoids, systematically detailing the standardized operational procedures from tissue isolation, enzymatic digestion, Matrigel embedding to primary culture. It analyzes key technical points in critical steps such as passaging and expansion, cryopreservation and revival, and explores how to stably obtain lung organoid models with typical cavity structures through precise control of operational steps.

Human fibroblast growth factor 10 (FGF-10) plays an irreplaceable core role in organoid culture systems, where it specifically regulates epithelial-mesenchymal interactions and is widely involved in the construction and homeostasis maintenance of various organoids, including those of the stomach, liver, lung, breast, and prostate.

The biological significance of Noggin as a key antagonist in the BMP signaling pathway.

Recurrent miscarriage refers to the loss of three or more fetuses before the 28th week of pregnancy. Since the risk of another miscarriage after two occurrences is similar to that after three, domestic experts recommend that attention and evaluation should be given after two miscarriages.

Pluripotent stem cells possess the potential for unlimited self-renewal and multidirectional differentiation, holding great promise in generating transplantable hematopoietic stem and progenitor cells, as well as various mature blood cells such as platelets and red blood cells.

The RSPO3 protein is a member of the R-spondin protein family, which includes four members: RSPO1, RSPO2, RSPO3, and RSPO4. The RSPO3 protein consists of approximately 270 amino acids, has a molecular weight of about 30 kilodaltons, and is a secreted glycoprotein.

Organoids are organ-specific cell aggregates derived from stem cells or multipotent progenitor cells, which essentially simulate the microenvironment of a specific organ in vitro to conduct three-dimensional cultivation of stem cells and their derived various cell types.

Small intestinal organoids are miniature tissue structures formed through the self-organization of adult stem cells from the small intestine under three-dimensional culture conditions. They can highly replicate the physiological structure and cellular composition of the intestinal epithelium, containing various cell types such as intestinal stem cells, Paneth cells, goblet cells, enteroendocrine cells, and absorptive enterocytes.