Precision Interaction, Unveiled at a Glance

TR-FRET Technology Empowers PROTAC/Molecular Glue Research

Kit Highlights:

Ready-to-use kit, no optimization required, direct detection saves time
Homogeneous assay, wash-free, simplified workflow, compatible with high-throughput
High sensitivity, low background, TR-FRET technology ensures stable and reliable signals
Multiple specifications available: 500T, 2500T, 10000T
Customization accepted

1. Binding Screening and Detection---Competitive Binding Assay

Human CRBN Ligand Screening Assay Kit

Detection Principle:

This kit efficiently and accurately screens compounds or ligands that specifically bind to CRBN, providing the inhibition constant Ki, offering a powerful tool for the design and optimization of PROTAC molecules.

Binding Activity and Inhibitor Validation:

Validation of Thalidomide-Ac binding to CRBN/DDB1. Results show that when protein concentration remains constant, the detection signal increases with higher Thalidomide-Ac concentrations. Lenalidomide, Pomalidomide, and PROTAC BET Degrader-1 can compete with Thalidomide-Ac for binding to CRBN/DDB1.

2. Binding Screening and Detection---Complex Formation Assay

These products are primarily used for studying the formation of PROTAC (Proteolysis Targeting Chimera)-mediated ternary complexes.

Catalog No.	Product Name
UA086022	Human VAV1/CRBN PROTAC Binding Kit
UA086021	Human BCL6/CRBN PROTAC Binding Kit
UA086019	Human BCL-XL/VHL PROTAC Binding Kit
UA086017	Human BCL-XL/CRBN PROTAC Binding Kit
UA086009	Human BRD4/CRBN PROTAC Binding Kit
UA086006	Human DDB1-CRBN&GSPT1 PROTAC Binding KIT
UA086005	Human DDB1-CRBN&IRAK4 PROTAC Binding KIT
UA086001	Human KRAS[G12C] /CRBN PROTAC Binding Kit
UA086002	Human KRAS[G12D] /CRBN PROTAC Binding Kit
UA086003	Human KRAS[G12V] /CRBN PROTAC Binding Kit
UA086004	Human KRAS[G13D] /CRBN PROTAC Binding Kit
UA085003	Human STAT6/CRBN PROTAC Binding Kit

3. Target Protein Degradation Detection

Product Name	Catalog No.	Specification
THUNDER™ Total IRAK4 TR-FRET Cell Signaling Assay Kit	KIT-IRAK4-500	500 points
THUNDER™ High Performance Total STAT6 TR-FRET Cell Signaling Assay Kit	KIT-HP-STAT6T-500	500 points
THUNDER™ Total BTK TR-FRET Cell Signaling Assay	KIT-BTKT-500	500 points
THUNDER™ Total GSPT1 TR-FRET Cell Signaling Assay Kit	KIT-GSPT1-500	500 points
THUNDER™ Total BRD4 TR-FRET Cell Signaling Assay Kit	KIT-BRD4-500	500 points
THUNDER™ Total BTK TR-FRET Cell Signaling Assay Kit	KIT-BTKT-500	500 points

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