Technical Articles

The main challenges of ACVR2A kinase activity assays lie in the expression system and reaction components. This article reviews the QC evidence of baculovirus-derived recombinant ACVR2A, ADP-Glo reaction parameters, standard curves, and troubleshooti

The main challenges of CHI3L1 binding assays are glycosylation-dependent epitopes and endotoxin interference. This article reviews the QC evidence of HEK293-expressed recombinant CHI3L1, platform parameters for ELISA, SPR and cell-based assays, and t

A systematic review of the prolactin receptor (PRLR), a class I cytokine receptor family member that transmits prolactin signals via the JAK2-STAT5 pathway: receptor structure and isoforms, signaling hierarchy, physiological functions, and the toolki

This article focuses on the technical principles and preparation strategies of recombinant proteins, systematically elaborating on their central role in life science research and biopharmaceutical development. It analyzes key technical elements such as the selection of expression systems, tag design, and purification strategies, and explores the broad applications of recombinant proteins in structural and functional studies, drug screening, and the development of diagnostic reagents.

Matrix metalloproteinase-9 (MMP-9, gelatinase B) is a member of the zinc-dependent endopeptidase family, capable of degrading various substrates in the extracellular matrix (ECM), including type IV collagen and gelatin.

Stem cell factor (SCF, also known as Kit ligand or mast cell growth factor) is a type I transmembrane protein, whose soluble form is produced by proteolytic cleavage and is the only known ligand for the c-Kit receptor (CD117).

Interleukin-21 receptor (IL-21R) is a member of the type I cytokine receptor family, forming a high-affinity heterodimeric receptor complex with the common gamma chain (γc) to mediate IL-21 signal transduction.

This article systematically introduces the molecular characteristics of recombinant GPX4 protein with dual Flag and His tags, its expression and purification strategies, as well as its key applications in elucidating the mechanisms of ferroptosis, screening inhibitors, and enzyme activity assays, providing core experimental materials for researchers in the fields of cell biology and drug development.

Noggin is a secreted glycoprotein and a key morphogen antagonist that regulates embryonic development and organ formation.

Furin/PCSK3 His Tag is a key protease tool protein expressed and purified through recombinant technology. Furin (also known as Proprotein Convertase 3 or PACE) is the most representative member of the proprotein convertase family, belonging to the calcium-dependent serine proteases.