Uprotein: Precision Empowerment, Driving Scientific Breakthroughs

Uprotein: Precision Empowerment, Driving Scientific Breakthroughs

In modern biomedical research, protein tag technology has become an indispensable tool, widely utilized in protein expression, purification, detection, and functional studies. Uprotein offers a diverse range of high-quality tagged proteins, encompassing His, FLAG, Avi, and other tags, to cater to varied research needs. These tagged proteins can be classified into the following categories based on their functions and applications:

I. Affinity Purification Tags

Affinity purification tags enable efficient purification of target proteins by binding to specific ligands or resins. Uprotein provides the following affinity purification tags:

• His Tag: Composed of six histidine residues, this tag forms coordination bonds with metal ions (e.g., Ni²⁺ or Co²⁺), enabling high-efficiency affinity purification. Its advantages include a small tag size (~0.84 kDa), minimal impact on protein structure and function, and mild purification conditions.

• GST Tag: Glutathione S-transferase, with a molecular weight of approximately 26 kDa, specifically binds to glutathione resins. It enhances protein solubility and facilitates purification, yielding high-purity products suitable for downstream experiments.

• MBP Tag: Maltose-binding protein, with a molecular weight of approximately 40 kDa, significantly improves the solubility and stability of target proteins, making it ideal for eukaryotic protein expression and purification.

II. Immunological Detection Tags

Immunological detection tags are recognized by specific antibodies for protein detection and quantitative analysis. Uprotein offers the following immunological detection tag:

• FLAG Tag: Comprising eight amino acids (DYKDDDDK), this tag exhibits high affinity and specificity, suitable for experiments such as Western Blot, immunoprecipitation, and flow cytometry.

III. Specialized Functional Tags

Specialized functional tags excel in specific experimental contexts, providing unique functional support. Uprotein provides the following specialized functional tags:

• Avi Tag: Enables covalent biotinylation of the tag's lysine residue via biotin ligase, offering high specificity and stability for protein purification and interaction studies.

• Biotin Tag: Leverages the ultra-strong binding between biotin and streptavidin for efficient protein immobilization and detection, applicable in SPR experiments and protein interaction studies.

• Fc Tag: Derived from the Fc fragment of immunoglobulins, this tag enhances protein stability and half-life, useful for cellular localization and functional studies.

One-Stop Service and Comprehensive Support

Uprotein offers a one-stop service suite encompassing prokaryotic, yeast, insect cell, and mammalian cell (CHO, 293) expression platforms, catering to diverse protein expression needs. Additionally, we provide customized protein services, tailoring the most suitable expression strategies to ensure efficient and stable protein production. With its advanced multi-platform systems and expert technical support, Uprotein is committed to empowering researchers and biotechnology enterprises in obtaining ideal protein products, advancing biotechnology, and making protein expression challenges a thing of the past.

Contact Us Today and Embark on Your Protein Expression Journey!