Efficient and Precise, One-Step Solution丨Extraction-free One-Step RT-qPCR Kit (SYBR Green/TaqMan Probe) Empowers Research and Diagnostics

Efficient and Precise, One-Step Solution丨Extraction-free One-Step RT-qPCR Kit (SYBR Green/TaqMan Probe) Empowers Research and Diagnostics

Introduction

In fields such as molecular biology research, pathogen detection, and gene expression analysis, reverse transcription quantitative polymerase chain reaction (RT-qPCR) is the gold standard technology due to its high sensitivity, specificity, and quantitative capabilities. However, traditional RT-qPCR workflows are cumbersome, particularly the RNA extraction process, which is time-consuming, labor-intensive, and prone to sample loss or degradation, resulting in low throughput.

To address these challenges, we introduce the Extraction-free One-Step RT-qPCR Kit (SYBR Green/TaqMan Probe). This kit integrates RNA extraction, reverse transcription, and qPCR amplification into a single step. After cell washing, samples are directly lysed, and the lysate is used as the template for RT-qPCR. The entire process—from cell preparation to PCR completion—can be finished in under two hours with standardized procedures, significantly reducing detection time, minimizing variables, increasing throughput, and boosting experimental efficiency.

Why Choose Our Extraction-free One-Step RT-qPCR Kit?

Extraction-free, Time-Saving, and Effortless

• Direct lysis of samples: Eliminates separate RNA extraction steps; simply lyse samples and proceed to RT-qPCR, reducing and contamination risks.

High Sensitivity and Specificity

• TaqMan Probe Method: Utilizes TaqMan probe technology for superior specificity, ideal for targets with high background or homologous sequences (e.g., viral typing, SNP analysis).
• SYBR Green Method: Employs highly sensitive fluorescent dye, offering cost-effectiveness for high-throughput screening or gene expression profiling.

User-Friendly Workflow

• No nucleic acid extraction, centrifugation, DNase digestion, or enzyme inactivation required.

Optimized Specificity

• The reaction system is rigorously optimized and validated to prevent non-specific amplification.

Product Catalog Numbers

Extraction-free One-Step RT-qPCR Kit (SYBR Green) | Cat. No.: UA079018

Extraction-free One-Step RT-qPCR Kit (TaqMan Probe) | Cat. No.: UA079019

Performance Validation

SYBR Green Method Validation (Gene Expression Detection)

HEK293 cells were enzymatically digested, washed with DMEM, counted, and distributed into six wells of a 96-well plate at cell densities of 15,000; 5,000; 1,667; 550; 183; and DMEM blank control. After centrifugation and supernatant removal, cells were lysed and subjected to RT-qPCR as per the kit instructions. Dark blue, light blue, dark green, light green, dark yellow, and light yellow bars represent the six sample groups. Panels A and C show amplification results for the UA kit and a domestic competitor’s product, respectively. Panels B and D display their corresponding melt curves. The UA kit demonstrates superior detection sensitivity and specificity compared to the competitor’s product.

TaqMan Probe Method Validation (Gene Expression Detection)

HEK293 cells were enzymatically digested, washed with DMEM, counted, and distributed into eight wells of a 96-well plate at cell densities of 15,000; 5,000; 1,667; 550; 183; 61; 20; and DMEM blank control. After centrifugation and supernatant removal, cells were lysed and subjected to RT-qPCR. The left-to-right order represents the eight sample groups. The negative control showed no amplification, while the 20-cell group exhibited faint signals at cycle 39. The remaining six groups showed clear amplification with excellent gradient linearity (Y-axis: Rn).

The Extraction-free One-Step RT-qPCR Kit redefines the convenience and efficiency of nucleic acid detection, making it an ideal choice for researchers and clinical diagnostic laboratories. Whether responding to emerging infectious diseases or conducting routine gene analysis, this kit ensures "one-step precision and efficiency"!

Simplify your experiments, trust your results!