"Universal Key" for Kinase Assays! ADP-Glo™ Kinase Assay, the Gold Standard in Homogeneous Luminescent Technology for High-Throughput Screening

In the field of biomedical research, kinases, as key regulators of cellular signaling, play a crucial role in fundamental biological and medical research, drug development, and clinical translation. Traditional kinase detection methods have numerous limitations, such as background interference in antibody-based assays, detection blind spots for low-activity kinases in ATP consumption assays, and experimental errors caused by multi-step operations, all of which impact kinase-related research. The ADP kinase detection kit, with its unique detection principle, outstanding performance, and broad application prospects, is gradually becoming the preferred tool for kinase detection.

The Ultimate Showdown: How ADP Kinase Detection Outperforms All

Why is ADP Detection the "Master Key"?

1. Universal Kinase Detection Platform Based on ADP Detection

Core Principle: From "Specific Detection" to "Common Capture"—Directly detecting the common product of all kinase reactions—ADP:

Step1: Background Clearance → Specific degradation of remaining ATP post-kinase reaction;

Step2: Signal Conversion → Conversion of generated ADP back to ATP;

Step3: Quantitative Analysis → Detection of newly generated ATP using a luciferase system, where luminescence intensity is proportional to ADP amount (i.e., kinase activity).

2. Comprehensive Validation of UA-Glo Kit Performance:

The EC₅₀ values of the compound staurosporine (STSP) for different kinases (e.g., PKA, CDK2/CycE1, SRPK1) were consistent with literature reports.

Platform Advantages

✅ Universal Applicability: Suitable for all enzymes generating ADP, including protein kinases, lipid kinases, sugar kinases, and ATPases;

✅ Easy Operation: Pre-mixed reagents, two-step process, greatly simplifying workflows

✅ High Sensitivity + Wide Detection Range: Detects as low as 0.25 pmol ADP, with ATP working concentration ranging from 1μM to 1 mM

✅ Stable Signal + High Tolerance: Stable luminescence signal for 0.5-12 hours; homogeneous detection method supports high-throughput screening, no strict timing required, reducing experimental errors, and compatible with high-throughput screening;

✅ Accurate Distinction of Inhibitor Types: Effectively distinguishes ATP-competitive and non-competitive inhibitors, providing more precise data for drug screening.

Full Coverage of Scenarios: A Versatile Tool from Basic Research to Drug Development

① High-Throughput Drug Screening (HTS)

Homogeneous detection, stable signal, enabling rapid preliminary screening of kinase inhibitors from hundreds of thousands of compound libraries.

② Kinase Inhibitor Characterization

Wide dynamic range, distinguishing ATP-competitive and non-competitive inhibitors, enabling IC₅₀ determination, selectivity analysis, and structure-activity relationship (SAR) studies of lead compounds.

③ ATPase Activity Analysis

Supports high ATP concentration detection, suitable for studying the activity of any enzyme hydrolyzing ATP to ADP (ATPases), such as Na+/K⁺ pumps, Ca²⁺ pumps, etc.

④ Signaling Pathway Analysis

Can detect activity changes of key kinases in pathways such as MAPK, PI3K/AKT, DDR, etc.

⑤ Emerging Fields

Viral Research: Detects interactions between viral proteins (e.g., SARS-CoV-2) and host kinases, accelerating antiviral drug development.

UA-Glo Facilitates Drug Development for Hot Targets: The ActRIIA/ActRIIB Enzyme Activity Inhibitor Screening Kit (Catalog No. UA080465) is now available, designed for high-throughput screening, enabling direct IC₅₀ determination of candidate compounds on ActRIIA/ActRIIB kinase activity

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Platform Information

Detection Equipment: Chemiluminescence reader, multifunctional microplate reader

Technical Advantages: Universal, sensitive, safe, stable

Applications: Kinase/ATPase activity detection, high-throughput screening, inhibitor characterization

U-Protein will continue to focus on technological innovation, providing ready-to-use UA-Glo kits, accompanying protocols, customized experimental solutions, and full technical support, offering "worry-free, efficient, and precise" detection solutions to accelerate innovative research and drug development!