Biotinylated Siglec-2 His&Avi Tag Protein, Human

1μg (R: reducing condition, N: non-reducing condition).

The purity of Biotinylated Siglec-2 His&Avi Tag Protein, Human is more than 95% determined by SEC-HPLC.

2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 1ug Biotinylated Siglec-2 His&Avi Tag Protein, Human, (Cat. No. UA011007) and unlabel respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with competitor respectively (Fig. D). APC signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.

Immobilized Biotinylated Siglec-2 His&Avi Tag Protein， Human
(Cat. No. UA011007) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Anti-Human CD22 Monoclonal Antibody(Pinbio) with EC50 of 1.59-2.04ng/ml.