Cullin4A-RBX1-DDB1-CRBN Protein

生物活性

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  The Cullin4A-RBX1-DDB1-CRBN activity was measured by protein-tracer binary binding assay using HTRF technology.
  

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  The Cullin4A-RBX1-DDB1-CRBN activity was measured by PROTAC-mediated Ternary Complex Formation using HTRF technology. The dose response curve of dBET1 in the Ternary Assay exhibited a bell-shaped curve, a hook effect and a peak.
  

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  The Cullin4A-RBX1-DDB1-CRBN activity was measured by WB analysis for target protein ubiquitination assay. The Ubiquitinated BRD4(D1+D2) bands increased with time.
  

电泳(SDS-PAGE)

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  Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl) 

  Lane 1: Cullin4A-RBX1-DDB1-CRBN complex under reducing condition (Sample loaded: 2 μg) 

  Lane 2: Cullin4A-RBX1-DDB1-CRBN complex under reducing condition (Sample loaded: 4 μg)
  

体积排阻色谱(SEC-HPLC)