Immobilized ADORA2A (with nine mutations) Flag-His-EGFP-strep tag Protein, Human (Detergent) (Cat. No. UA060006) at 5 μg/mL (100 μL/well) on a Nickel Coated plate can bind Mouse Anti-ADORA2A with EC50 of 1.89-4.07ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured ADORA2A (with nine mutations) Flag-His-EGFP-strep tag Protein, Human (Detergent) (Cat. No. UA060006), can bind Mouse Anti-ADORA2A with an affinity constant of 2.62nM as determined in SPR assay.
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