5e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug Alexa Fluor 647 Labeled-Siglec-2 His Tag Protein, Human, (Cat. No. UA011196) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 5e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug Siglec-2 His Tag Protein, Human (Cat. No. UA010679) and competitor respectively (Fig. D and E). APC signal was used to evaluate the binding activity.
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