PE-Labeled Siglec-2/CD22 His&Avi Tag Protein, Human

流式分析

• 

• 

  2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug PE-Labeled Siglec-2 His&Avi Tag Protein, Human, (Cat. No. UA011228) and unlabel respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity.
  2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with competitor respectively (Fig. D). APC signal was used to evaluate the binding activity.
  2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.