Biotinylated Siglec-2 Fc&Avi Tag Protein, Human

1μg (R: reducing conditions, N: non-reducing conditions).

2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug Biotinylated Siglec-2 Fc&Avi Tag Protein, Human, (Cat. No. UA010426) and unlabel respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with competitor respectively (Fig. D). APC signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.

Immobilized Anti-Human CD22 Monoclonal Antibody(Pinbio) at 1.0μg/mL (100μL/well) can bind Biotinylated Siglec-2 Fc&Avi Tag Protein, Human (Cat. No. UA010426) with EC50 of 1.92-2.95ng/mL.