What are the types of protein purification?

2026-03-20

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Common chromatography methods for protein purification:

Affinity chromatography: Enriches target proteins via tags or specific binding.
Ion-exchange chromatography: Separates proteins based on charge differences.
Gel filtration chromatography: Fractionates proteins according to molecular size.
Hydrophobic interaction chromatography: Purifies proteins by hydrophobic differences.

These processes help remove impurities, exchange buffers, improve protein quality, and reduce endotoxin levels. Multi-step combinations further enhance purity and bioactivity.