What is the CIPP purification strategy?

2026-03-24

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The CIPP chromatography strategy (Capture-Intermediate Purification-Polishing) is a classic three-step method for protein purification.

Capture: Affinity chromatography is used to rapidly isolate and concentrate the target protein and stabilize the product.
Intermediate Purification: Ion-exchange chromatography and hydrophobic interaction chromatography are applied to remove bulk impurities such as host proteins and nucleic acids.
Polishing: Gel filtration chromatography is used to remove residual trace impurities, achieving final high purity.