Membrane protein purification is a technique for isolating and purifying cell membrane-associated proteins. First, cell membranes are disrupted to lyse the sample; dedicated detergents are then used to solubilize hydrophobic membrane proteins. Subsequent steps, including chromatography and affinity enrichment (e.g., His Tag), are employed to remove lipids, contaminating proteins and other impurities. The final product is high-purity, highly active target membrane proteins, supporting applications such as target research, antibody preparation, and recombinant protein reagent development.