Tagged protein affinity chromatography is a common protein purification technique. Target proteins are fused with specific tags such as His Tag and GST Tag through genetic engineering. The dedicated ligands on the chromatography resin specifically adsorb these tags to precisely capture the target protein and remove impurities and contaminating proteins. The bound protein is dissociated using an elution buffer, allowing rapid acquisition of high-purity and highly active protein. With simple operation and strong specificity, it is widely used in the preparation of scientific research reagents and recombinant proteins.