Discovery & Hypothesis Generation: Identify candidate pathways and key molecules via unbiased screening and literature mining, then formulate scientific hypotheses.

Validation & Confirmation: Verify the causal regulatory relationship between molecules and pathways through pathway activity assays and gene functional manipulation.

Mechanism & Interaction Elucidation: Elucidate molecular interaction patterns and signal transduction mechanisms using protein-protein interaction assays, transcriptional regulation analyses, and structural biology approaches, ultimately constructing a complete regulatory pathway mode