Why Top Laboratories Are Using This ADP Kinase Assay Kit?

In the fields of life sciences and drug development, precise detection of kinase activity is crucial for understanding cellular signaling, metabolic regulation, and disease mechanisms. ADP kinases (such as AMPK, PI3K, etc.) are key regulators of energy metabolism and cellular stress responses.

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Recent Advances
In the fields of life sciences and drug development, precise detection of kinase activity is crucial for understanding cellular signaling, metabolic regulation, and disease mechanisms. ADP kinases (such as AMPK, PI3K, etc.) are key regulators of energy metabolism and cellular stress responses. Their activity detection is of great significance to research in cancer, diabetes, neurodegenerative diseases, and other areas.
The ADP kinase assay kit, with its innovative technical design, superior detection performance, and convenient operating procedures, provides strong support for your scientific research!
Detection Principle
Product Advantages
  • ATP concentrations ranging from 1 μM to 1 mM can be used in experiments, allowing differentiation between competitive and non-competitive kinase inhibitors.
  • Extremely high sensitivity and a broader dynamic range: ADP as low as 25 pmol can be detected.
  • Stable signal: Fluorescent signals can be detected for 5 to 12 hours.
  • Convenient experimental procedures.
  • Suitable for high-throughput detection.
Applications
  • Kinase activity detection (quantitative and qualitative).
  • Kinase drug screening and profiling.
  • ATPase activity detection.
Related Products

Catalog Number

Product Name

UA070101

UA-Glo Kinase ADP Assay

UA079021

UA-Glo Kinase ADP Max Assay Kit

 

Product Data
Detection of EC50 for Compounds H-89 and PKI:
Using the market-leading brand PC, the EC50 values for H-89 were measured under the same experimental conditions with ATP concentrations of 10 μM and 100 μM, resulting in EC50 values of 65 nM and 567 nM, respectively. These values are consistent with those obtained using the UA-Glo Kinase ADP Detection Kit.
Detection of EC50 for Compound Staurosporine on Three Kinases:
dose-response curves and EC50 results for staurosporine (STSP) at different ATP concentrations are shown in the figure above. The EC50 values are consistent with those reported in the literature.
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