UA-Blue SEAP Assay Kit: A New Domestically Produced Benchmark with 2x Sensitivity and 2x Speed

One-Sentence Conclusion

UA-Blue doubles the sensitivity of SEAP reporter gene detection, halves the reaction time, and requires no cell lysis, making it the preferred tool for promoter studies, signaling pathway screening, and high-throughput drug discovery.

Why is SEAP the Top-Tier Reporter Gene?

• Non-destructive sampling: Cells continue normal growth, allowing repeated monitoring of the same sample.

• Near-zero background: Endogenous phosphatases are inactivated at 55 °C for 10 minutes, resulting in cleaner data.

Three Core Advantages of UA-Blue

Experimental Snapshot: THP1-Dual Model

• Cell density: 5×10⁵ cells/mL

• Stimulant: Pam3CSK4, 3 µg/mL, 24 h
  
  

• Reading: 650 nm, completed within 12 minutes
  
  

Result: UA-Blue shows ≥2.1× signal intensity in the low-to-medium enzyme range compared to imported reagents, with CV <5%.

5 Major Application Scenarios (UA-Blue SEAP Detection Kit)

• Quantification of gene promoter/enhancer activity
  
  

• High-throughput screening of GPCR-targeting drugs
  
  

• Mapping of viral infection signaling pathways
  
  

• Discovery of immune response agonists/inhibitors
  
  

• Long-term tracking of low-expression cell models
  
  

Ready-to-Use Protocol: Get Started in 1 Minute

1. Take 20 µL of culture supernatant → transfer to a 96-well plate
   
   

2. Add 180 µL of UA-Blue working solution (ready-to-use, no preparation)
   
   

3. Perform kinetic reading at 37 °C for 5–15 minutes → Done!

Product Information

• Catalog No.: UA079029

• Product Name: UA-Blue SEAP Detection Kit

• Sizes: 100 rxn / 500 rxn

• Storage: 4 °C, protected from light, stable for 12 months

• Website: www.ua-bio.com