Selected Proteases for Peptide Mapping Analysis

The Process of Peptide Coverage by Mass Spectrometry/Determination of the Complete Peptide Map Sequence:

• Use proteases such as PNGase F, Trypsin, Chymotrypsin, Asp-N, Glu-C, Lys-C, and Lys-N to respectively digest and identify the target protein.

• Analyze the samples by nano LC-MS/MS or UV LC-MS/MS.

• Compare the obtained peptide coverage/complete peptide map sequence with the theoretical sequence you provided.

• The comparison results

Data Sharing:

Recombinant Trypsin (Mass Spectrometry Grade)：

The sequence coverage of the BSA sample after digestion by Trypsin and analysis by LC-MS/MS is 99.7%.

Recombinant Endoproteinase Lys-C：

Compared with the imported brand of Lys-C, the peptide map is basically the same, and there are basically no differences in common post-translational modifications (PTMs).

V8 Protease (Glu-C):

The sequence coverage of the BSA sample after digestion by Glu-C and analysis by LC-MS/MS is 98.3%.

Recombinant α-Chymotrypsin:

After proteolytic digestion, compared with the chymotrypsin of mainstream brands, the peptide map and sequence coverage are basically the same.

Metalloprotease (Asp-N):

The sequence coverage of the BSA sample after digestion by AspN-1 and analysis by LC-MS/MS is 91.8%.

Glycerol-free PNGase F: