Alexa Fluor 647-Labeled Her2 His Tag Protein: A Vital Tool for Precise Detection and Cutting-Edge Research
Human epidermal growth factor receptor 2 (Her2) is a key member of the ErbB receptor tyrosine kinase family, playing a central role in regulating cell proliferation, differentiation, and survival signaling pathways.
- Recent Advances
- Product Information
I. Biological Characteristics and Clinical Significance of Her2 Protein
Human Epidermal Growth Factor Receptor 2 (Her2), a key member of the ErbB receptor tyrosine kinase family, plays a central role in regulating cell proliferation, differentiation, and survival signaling pathways. Her2 gene amplification or protein overexpression is observed in approximately 20% of breast cancers and certain solid tumors such as gastric and ovarian cancers. Its abnormal activation is closely associated with strong tumor invasiveness, rapid progression, and poor prognosis. Her2 serves not only as an important prognostic marker but also as a core therapeutic target for treatments like trastuzumab, pertuzumab, and antibody-drug conjugates. Therefore, precise quantification and localization analysis of Her2 protein expression levels are fundamental for guiding clinical diagnosis, treatment decisions, and prognosis evaluation.
II. Technical Features of Alexa Fluor 647-Labeled Her2 His Tag Protein
Alexa Fluor 647-Labeled Her2 His Tag Protein is a genetically engineered and chemically conjugated composite recombinant protein that integrates multiple technical advantages:
1. Retention of Functional Domains: The protein contains the extracellular ligand-binding domain of Her2, preserving the bioactive conformation for interactions with antibodies (e.g., trastuzumab) or natural ligands.
2. High-Efficiency Affinity Purification Tag: The C- or N-terminal fused polyhistidine tag (His Tag) enables one-step high-efficiency purification via metal chelate chromatography (e.g., nickel column), ensuring high-purity protein with excellent recovery rates.
3. High-Performance Fluorescent Labeling: The covalently linked Alexa Fluor 647 dye is a classic far-red fluorescent dye with long excitation/emission wavelengths (~650/670 nm), strong photostability, high quantum yield, and minimal spectral overlap with other common fluorescence channels (e.g., FITC, PE). It is ideal for multicolor flow cytometry, high-resolution imaging, and deep tissue detection.
III. Core Application Areas
1. Flow Cytometry Analysis
- Standard Curve Establishment and Absolute Quantification: As a quantitative standard, it is used to establish standard curves for Her2 antigen expression levels on cell surfaces, enabling absolute quantification of Her2 expression and providing precise criteria for clinical subtyping (e.g., Her2-low expression).
- Multicolor Immunophenotyping: Combined with other cellular markers (e.g., ER, PR, Ki67), it enables multiparameter immunophenotyping at the single-cell level, revealing tumor heterogeneity.
- Antibody Binding Competition and Blocking Assays: Used to evaluate the binding affinity of candidate therapeutic antibodies (e.g., novel monoclonal or bispecific antibodies) and their competitive inhibition of known antibodies (e.g., trastuzumab).
2. Immunofluorescence and Confocal Microscopy Imaging
- In Situ Localization in Cells and Tissues: Used for immunofluorescence staining of cell slides or tissue sections to visualize the distribution density, polarity, and internalization of Her2 on cell membranes.
- Colocalization and Interaction Studies: Combined with other organelle or signaling protein markers, it investigates the colocalization of Her2 with downstream signaling molecules or endocytosis-related proteins.
3. Biosensor and In Vitro Diagnostic Development
- Diagnostic Reagent Calibration: As a standard antigen, it calibrates Her2 detection-based immunoassay kits (e.g., ELISA, chemiluminescence), ensuring accuracy and comparability of results.
- Development of Novel Detection Technologies: Used to develop rapid Her2 detection platforms based on surface plasmon resonance or bio-layer interferometry.
4. Drug Screening and Functional Studies
- Antibody Drug Potency Evaluation: As a target antigen, it facilitates high-throughput screening or evaluation of the binding activity and functional efficacy of Her2-targeting antibody drugs, antibody-drug conjugates, or small-molecule inhibitors.
- Signaling Pathway Research Models: Can serve as an exogenous stimulator or blocker to study the effects of Her2 pathway activation or inhibition on downstream cellular behaviors.
IV. Summary and Outlook
Alexa Fluor 647-Labeled Her2 His Tag Protein integrates high-purity antigen, convenient purification tags, and high-performance fluorescent reporters, serving as an efficient and reliable standardized tool for Her2 quantification, mechanistic research, and drug development. Its applications span the entire spectrum from basic biological exploration to clinical translational research. With the advancement of precision medicine, particularly the innovation in treatment strategies for Her2-low breast cancer, the demand for finer and more quantitative Her2 protein detection is growing. In the future, such standardized fluorescent-labeled protein tools, combined with automated analysis platforms and AI-based image analysis algorithms, will play an increasingly critical role in advancing tumor precision subtyping, guiding personalized therapy, and accelerating novel targeted drug development.
V. Which Manufacturers Provide Alexa Fluor 647-Labeled Her2 His Tag Protein?
Nanjing UniLove Biotech's independently developed Alexa Fluor 647-Labeled Her2 His Tag Protein, Human (Catalog No.: UA011370) is an innovative, ready-to-use monomeric detection probe that combines high specificity, ultra-strong fluorescence signals, and versatile applications. This product features the extracellular domain of human Her2 (ERBB2, EGFR2) fused with a His tag at the C-terminus and covalently labeled with the high-performance Alexa Fluor 647® fluorescent dye. Her2 is a key therapeutic target and prognostic marker for various solid tumors, including breast and gastric cancers. This product provides an exceptional monomeric tool for high-sensitivity detection, quantitative analysis, and molecular interaction studies.
| Core Product Advantages |
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| Precise Monomeric Probe Design: The monomeric His tag design enables monovalent binding, avoiding potential aggregation or signal amplification effects caused by multivalent probes. It is suitable for experiments requiring precise measurement of affinity (e.g., KD values), competitive binding analysis, or investigation of interactions between single Her2 receptors and ligands. |
| Ultra-Sensitive Fluorescence Signal: The Alexa Fluor 647® dye exhibits extremely high fluorescence intensity, exceptional photostability, and outstanding resistance to quenching. Its emission wavelength in the far-red region (~668 nm) effectively avoids autofluorescence from most cell and tissue samples, delivering superior signal-to-noise ratios and detection sensitivity in flow cytometry, tissue imaging, and live-cell dynamic monitoring. |
| Rigorous Quality Control and Consistency: The product undergoes meticulous labeling process control and multi-dimensional quality checks to ensure uniform and stable dye-to-protein labeling ratios (F/P ratios) while fully retaining Her2's antigen-binding activity. Strict batch-to-batch quality control guarantees reliable and reproducible experimental results. |
| Clear and Cutting-Edge Applications: |
| - High-Sensitivity Her2 Expression Quantification: Directly used in flow cytometry to precisely quantify Her2 expression levels on tumor cell lines, primary tumor cells, or circulating tumor cells (CTCs), even in low-expression samples. |
| - Antibody Drug Development and Evaluation: As a standardized fluorescent ligand, it is used on biosensor platforms to assess the binding kinetics, affinity, and competitiveness of therapeutic antibodies (including trastuzumab biosimilars and novel bispecific antibodies). |
| - Live-Cell Imaging and Endocytosis Tracking: Leveraging its excellent optical properties, it enables real-time, dynamic tracking of Her2 antibody-receptor complex binding, aggregation, and internalization in live cells. |
| - Multicolor Flow Cytometry and Tissue Imaging: Its far-red emission facilitates combination with other common fluorescent dyes (e.g., FITC, PE) for multicolor analysis. |
Nanjing UniLove Biotech is dedicated to providing precise, high-performance molecular imaging and interaction analysis tools for tumor-targeted therapy, antibody engineering, and translational medicine research. For detailed technical parameters (e.g., F/P ratios), application protocols on specific platforms (e.g., SPR/BLI), or comparative consultations with other detection formats for Alexa Fluor 647-Labeled Her2 His Tag Protein, Human (Catalog No.: UA011370), please feel free to contact us.
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