When PD-L1 His tag, Human is immobilized at 2.8µg/mL, it binds to PD-1 Fc Chimera, Human with an EC50 of 4.334-5.695µg/mL.
PD-L1 His Tag, Human (Cat. No.UA010001) captured on CM5 chip via anti-His antibody, can bind PD-1 Fc Chimera, Human (Cat. No. UA010002) with an affinity constant of 0.1836 μM as determined in a SPR assay (Biacore T200).
Loaded PD-1 Fc Chimera, Human (Cat. No. UA010002) on Protein A Biosensor, can bind PD-L1 His Tag, Human (Cat. No. UA010001) with an affinity constant of 1.3 μM as determined in BLI assay.
电泳(SDS-PAGE)
PD-L1 His Tag, Human,1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.
ELISA
Immobilized PD-L1 His Tag Protein, Human (Cat. No. UA010001) at 1.0μg/mL (100μL/well) can bind S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-119-286) (Cat. No. S0B2287) with EC50 of 0.89-1.21ng/mL.
评论(0)