PD-1 Fc Chimera Protein, Human

PD-1 Fc Chimera, Human 1μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

Immobilized PD-L1 His Tag, Human (Cat. No. 010001) at 2.8μg/mL (100μL/well) can bind PD-1 Fc Chimera, Human (Cat. No. 010002) with an EC50 of 4.33-5.70µg/mL.

Immobilized Biotinylated PD-L2 Avi&His Tag Protein, Human (Cat. No. UA010568) at 1.0μg/mL (100μL/well) on streptavidin precoated (0.5 μg/well) plate can bind PD-1 Fc Chimera Protein, Human (Cat. No. UA010002) with EC50 of 2.83-4.59 ng/mL.

Immobilized Biotinylated PD-L1 Avi&His Tag Protein, Human (Cat. No. UA010541) at 1.0μg/mL (100μL/well) Streptavidin precoated(0.5ug/well) can bind PD-1 Fc Chimera Protein, Human (Cat. No. UA010002) with EC50 of 4.31-7.26ng/mL.

PD-L1 His Tag, Human (Cat. No. UA010001) captured on CM5 chip via anti-His antibody, can bind PD-1 Fc Chimera, Human (Cat. No. UA010002) with an affinity constant of 0.1836 μM as determined in a SPR assay (Biacore T200)

Loaded PD-1 Fc Chimera, Human (Cat. No. UA010002) on Protein A Biosensor, can bind PD-L1 His Tag, Human (Cat. No. UA010001) with an affinity constant of 1.3 μM as determined in BLI assay.

Anti-His antibody Immobilized on CM5 Chip captured PD-L2/B7-DC His Tag, Human (Cat. No. UA010312), can bind PD-1 Fc Chimera, Human (Cat. No. UA010002) with an affinity constant of 87.22 nM as determined in SPR assay.