PD-L1 Fc Chimera, Human, 2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.
ELISA
Immobilized PD-1 His Tag, Human (Cat. No. abs05023) at 1.5μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human(Cat. No. abs05024) with an EC50 of 0.142-0.174µg/mL.
Immobilized PD-1 His Tag, Cynomolgus (Cat. No. UA010311)
at 2.0μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003)
with EC50 of 0.048-0.063μg/ml.
SPR
PD-L1 His Tag, Human (Cat. No.UA010001) captured on CM5 chip via anti-His antibody, can bind PD-1 Fc Chimera, Human (Cat. No. UA010002) with an affinity constant of 0.1836 μM as determined in a SPR assay (Biacore T200).
Loaded PD-L1 Fc Chimera, Human (Cat. No. UA010003) on Protein A Biosensor, can bind PD-1 His Tag, Human (Cat. No. UA010004) with an affinity constant of 1.6 μM as determined in BLI assay.
Protein A Chip captured PD-L1 Fc Chimera, Human (Cat. No.
UA010003), can bind PD-1 His Tag, Cynomolgus (Cat. No. UA010311) with an
affinity constant of 0.78 μM as determined in SPR assay.
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