PD-1 His Tag Protein, Human

Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 1.5μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with an EC50 of 0.141-0.174µg/mL.

Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 0.5μg/mL (100μL/well) can bind Nivolumab (starter-bio, Cat. No. S0B0566) with EC50 of 2.24-2.98 ng/mL.

Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 0.5μg/mL (100μL/well) can bind Pembrolizumab (starter-bio, Cat. No. S0B0233) with EC50 of 1.28-1.49 ng/mL.

PD-1 His Tag, Human ,1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.

PD-1 His Tag, Human (Cat. No. UA010004) captured on CM5 chip via anti-His antibody, can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with an affinity constant of 0.6644 μM as determined in a SPR assay (Biacore T200).

Loaded PD-L1 Fc Chimera, Human (Cat. No. UA010003) on Protein A Biosensor, can bind PD-1 His Tag, Human (Cat. No. UA010004) with an affinity constant of 1.6 μM as determined in BLI assay.