2e5 of transient transfected anti-BCMA ScFv CAR-293 cells were stained with 1ug BCMA/TNFRSF17 Fc Chimera Protein, Human, (Cat. No. UA010043) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti- BCMA ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
ELISA
Immobilized BCMA/TNFRSF17 Fc Chimera Protein, Human (Cat. No. UA010043) at 2.0μg/mL (100μL/well) can bind Biotinylated Anti-Human BCMA (Belantamab) with EC50 of 1.87-4.14 ng/ mL.
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