The purity of BCMA/TNFRSF17 His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
流式分析
2e5 of transient transfected anti-BCMA ScFv CAR-293 cells were stained with 0.1ug BCMA His Tag Protein, Human, (Cat. No. UA010083) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti- BCMA ScFv CAR-293 cells were stained with competitor respectively (Fig.D). APC signal was used to evaluate the binding activity.
2e5 of transient transfected anti- BCMA ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.
ELISA
Immobilized BCMA/TNFRSF17 His Tag Protein, Human (Cat. No. UA010083) at 2.0μg/mL (100μL/well) can bind Anti-Human BCMA (Belantamab) with EC50 of 0.68-0.88ng/mL.
Immobilized BCMA/TNFRSF17 His Tag Protein, Human (Cat. No. UA010083) at 2.0μg/mL (100μL/well) can bind Anti-Human BCMA (Belantamab), LotA with EC50 of 1.00-1.55ng/mL. LotB with EC50 of 1.15-1.66ng/mL.
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