Immobilized CD3 epsilon Mouse mAb (SDT-570-36) (Cat. No. S0B2258) at 2.0μg/mL (100μL/well) can bind CD3 epsilon Fc Chimera Protein, Human (Cat. No. UA010199) with EC50 of 0.55-1.14ng/ mL.
Immobilized CD3 epsilon Fc Chimera Protein, Human (Cat. No. UA010199) at 2 μg/mL (100 μL/well) can bind Human CD3 delta (Cat. No. S0A1042), His tag with EC50 of 3.8-4.6 μg/ ml.
Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 gamma Fc Chimera (Cat.No.UA010598) is immobilized at 1.0 μg/mL, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera(Cat. No. UA010199) that produces 50% of the optimal binding response is approximately 2.2-3.2 μg/mL.
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