FITC-Labeled CD19 Fc Chimera Protein, Human

Immobilized Anti-CD19 Antibody, Mouse Monoclonal at 0.5μg/mL (100μL/well) can bind FITC-Labeled CD19 Fc Chimera, Human (Cat. No. UA010211) with EC50 of 11.12ng/ml.

Immobilized Anti-CD19 Antibody, Mouse Monoclonal at 2μg/mL (100μL/well) can bind FITC-Labeled CD19 Fc Chimera, Human (Cat. No. UA010211) with EC50 of 0.43μg/ml.

1μg (R: reducing conditions).

2e5 of transient transfected anti-CD19 ScFv CAR-293 cells were stained with 0.1ug FITC - CD19 Fc Chimera Protein, Human, (Cat. No. UA010211) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). FITC signal was used to evaluate the binding activity.

2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with 0.01ug CD19 Fc Chimera Protein, Human (Cat. No. UA010042) (Fig. D). Alexa Fluor 647 signal was used to evaluate the binding activity.

2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with isotype and G4S Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.