2e5 of transient transfected anti-ROR1 ScFv CAR-293 cells were stained with 0.01ug ROR1 Fc Chimera Protein, Human, (Cat. No. UA010271) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti- ROR1 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
ELISA
Immobilized ROR1 Fc Chimera Protein, Human (Cat. No. UA010271) at 2.0μg/mL (100μL/well) can bind Biotinylated Anti-Human ROR1 (Zilovertamab) with EC50 of 4.38-7.57ng/mL.
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