PD-1 His Tag Protein, Mouse

1μg (R: reducing conditions, N: non-reducing conditions).

Immobilized PD-1 His Tag, Mouse (Cat. No. UA010292) at 2.0μg/mL (100μL/well) can bind PD-L1/B7-H1 Fc Chimera, Mouse (Cat. No. UA010259) with EC50 of 2.18-3.03μg/ml.

Protein A Chip captured PD-L1/B7-H1 Fc Chimera, Mouse (Cat. No. UA010259), can bind PD-1 His Tag, Mouse (Cat. No. UA010292) with an affinity constant of 0.42μM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured PD-1 His Tag, Mouse (Cat. No. UA010292), can bind PD-L2/B7-DC Fc Chimera, Mouse (Cat. No. UA010337) with an affinity constant of 0.31 μM as determined in SPR assay.