Immobilized EGF, Human (Cat. No. UA040003) at 2.0μg/mL (100μL/well) can bind EGFR His Tag, Human (Cat. No. UA010306) with EC50 of 8.03-10.32μg/ml.
Immobilized EGFR His Tag, Human (Cat. No. UA010306) at 0.5μg/mL (100μL/well) can bind Cetuximab (starter-bio, Cat. No. S0B0429) with EC50 of 1.07-1.33 ng/mL.
CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Human (Cat. No. UA010306) with an affinity constant of 2.01μM as determined in SPR assay.
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