CD30/TNFRSF8 His Tag Protein, Human

1μg (R: reducing conditions, N: non-reducing conditions).

2e5 of transient transfected anti-CD30/TNFRSF8 ScFv CAR-293 cells were stained with 0.01ug CD30/TNFRSF8 His Tag Protein, Human, (Cat. No. UA010362) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti- CD30/TNFRSF8 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

Immobilized CD30/TNFRSF8 His Tag, Human (Cat. No. UA010362) at 2μg/mL (100μL/well) can bind Anti-Human CD30 Monoclonal Antibody with EC50 of 4.66-6.44ng/ml.

Immobilized CD30/TNFRSF8 His Tag Protein, Human (Cat. No. UA010362) at 2μg/mL (100μL/well) can bind CD30 Ligand mFc Chimera Protein, Human (Cat. No. UA010378) with EC50 of 0.29-0.34μg/ml.