Syndecan-1/CD138 His Tag Protein, Human

1μg (R: reducing conditions, N: non-reducing conditions).

2e5 of transient transfected anti-Syndecan-1 ScFv CAR-293 cells were stained with 0.1ug Syndecan-1/CD138 His Tag Protein, Human, (Cat. No. UA010400) and unlabel respectively and unrelated protein (Fig. C and B and D), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor® 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Syndecan-1 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. F and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

Immobilized Syndecan-1/CD138 His Tag, Human (Cat. No. UA010400) at 2.0μg/mL (100μL/well) can bind CD138 Recombinant Rabbit mAb (SDT-R120) (Cat. No. S0B2159)  with EC50 of 2.13-3.30ng/mL.