CD27 Ligand/TNFSF7 Fc Chimera Protein, Human

1μg (R: reducing conditions, N: non-reducing conditions).

Immobilized CD27/TNFRSF7 His Tag, Human (Cat. No. UA010124) at 2.0μg/mL (100μL/well) can bind CD27 Ligand/TNFSF7 Fc Chimera, Human (Cat. No. UA010611) with EC50 of 5.89-20.94ng/mL.

Serial dilutions of V6 Anti-Human CD70 (Cusatuzumab) were added into CD27 Ligand/TNFSF7 Fc Chimera Protein, Human (Cat. No. UA010611): Biotinylated CD27/TNFRSF7 His Tag Protein, Human binding reactions. The half maximal inhibitory concentration (IC50) is 0.069μg/mL.

Protein A Chip captured CD27 Ligand/TNFSF7 Fc Chimera, Human (Cat. No. UA010611), can bind CD27/TNFRSF7 His Tag, Human (Cat. No. UA010124) with an affinity constant of 1.16μM as determined in SPR assay.