Siglec-2 His Tag Protein, Human

Immobilized Siglec-2 His Tag, Human (Cat. No. UA010679) at 2μg/mL (100μL/well) can bind Anti-Human CD22 Monoclonal Antibody with EC50 of 4.47-5.30ng/ml. 

2μg (R: reducing condition, N: non-reducing condition).

2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug Siglec-2 His Tag Protein, Human, (Cat. No. UA010679) and unlabel respectively (Fig. B and A), and non-transfected 293 cells were used as a control (Fig. C). APC signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with competitor respectively (Fig. D). APC signal was used to evaluate the binding activity. 2e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.