2e5 of transient transfected anti- Siglec-2 ScFv CAR-293 cells were stained with 0.1ug FITC-Labeled Siglec-2/CD22 Fc Chimera Protein, Human, (Cat. No. UA011266) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). FITC signal was used to evaluate the binding activity. 2e5 of transient transfected anti- Siglec-2 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
评论(0)